The Stomach

The Stomach

6 min read Updated Mar 26, 2026

The stomach is a muscular sac that serves as a holding tank and processing plant. It stores food, mixes it with acid and enzymes, and slowly releases the resulting slurry (chyme) into the small intestine. The stomach’s acid is so strong it could dissolve a nail - yet the stomach lining survives because of a constantly renewed mucus shield.

Stomach Anatomy

Anatomy of the stomach showing the cardia, fundus, body, and pylorus regions with the lower esophageal sphincter and pyloric sphincter labeled, plus rugae folds on the inner surface
Stomach anatomy showing the four regions and sphincters. Rugae (folds) on the inner surface flatten as the stomach expands. Credit: OpenStax Anatomy and Physiology 2e, CC BY 4.0

The stomach has four main regions:

  1. Cardia - where the esophagus connects; just below the lower esophageal sphincter
  2. Fundus - the upper dome that often collects swallowed air
  3. Body - the largest region; main site of mixing and digestion
  4. Pylorus - the lower region that connects to the duodenum via the pyloric sphincter

The inner surface is thrown into folds called rugae, which flatten as the stomach expands (like an accordion). This allows the stomach to stretch from ~50 mL (empty) to ~1-1.5 L (after a large meal).

Gastric Glands: The Acid Factory

Scattered across the stomach lining are millions of gastric pits - small depressions that lead down to gastric glands. These glands contain four specialized cell types:

Labeled diagram of stomach anatomy showing cardia, fundus, body, pylorus, and muscularis layers
Stomach anatomy with regions and muscle layers. Focus on: the four regions (cardia, fundus, body, pylorus), the three muscle layers, and the key cell types: parietal cells (HCl + intrinsic factor), chief cells (pepsinogen), G cells (gastrin). Credit: OpenStax College, CC BY 3.0
Cell TypeSecretionFunction
Parietal cellsHCl, intrinsic factorHCl activates pepsinogen, kills bacteria; intrinsic factor needed for B12 absorption in ileum
Chief cellsPepsinogen, gastric lipasePepsinogen is the inactive zymogen of pepsin; gastric lipase digests some fat
Mucous neck cellsAlkaline mucusCoats the stomach lining with a bicarbonate-rich barrier, protecting it from acid
G cellsGastrin (hormone)Stimulates parietal cells to produce more HCl; stimulates stomach motility

HCl: The Chemical Weapon

Parietal cells secrete HCl into the stomach lumen using the H+/K+ ATPase (proton pump). This pump exchanges H+ ions (into the lumen) for K+ ions (into the cell), using ATP. The result is a lumen pH of about 1.5-2 - acidic enough to kill most bacteria and denature proteins.

The HCl does three things:

  1. Activates pepsinogen into pepsin (the active protease)
  2. Denatures proteins - unfolds them, exposing peptide bonds to enzymatic attack
  3. Kills pathogens - acts as a first line of defense against ingested bacteria

Pepsinogen to Pepsin: The Zymogen Cascade Begins

Chief cells secrete pepsinogen, the inactive form of the protease pepsin. This is a critical safety mechanism - if chief cells secreted active pepsin, the enzyme would digest the cells that made it.

Once pepsinogen contacts HCl in the lumen, the acid cleaves off a small peptide fragment, converting pepsinogen into active pepsin. Pepsin then:

  • Digests proteins into shorter peptide fragments
  • Works optimally at pH ~2 (and is inactive above pH ~6)
  • Can activate more pepsinogen (positive feedback loop)

Intrinsic Factor: The B12 Key

Parietal cells also secrete intrinsic factor, a glycoprotein that binds vitamin B12 in the stomach. The intrinsic factor-B12 complex is absorbed later in the ileum (the last segment of the small intestine). Without intrinsic factor, B12 cannot be absorbed, which leads to a form of anemia caused by B12 deficiency.

Gastric Motility and Chyme Formation

The stomach’s three smooth muscle layers (an extra oblique layer compared to the rest of the GI tract) churn and mix food with gastric juice. After 2-6 hours of mechanical and chemical digestion, food is converted into a thick, acidic paste called chyme.

Chyme is released into the duodenum in small squirts through the pyloric sphincter. The rate of gastric emptying is regulated by:

  • Duodenal feedback - fat and acid in the duodenum slow gastric emptying (via CCK and secretin)
  • Gastrin - speeds up gastric emptying
  • Volume and composition - liquids empty faster than solids; carbohydrates faster than proteins; fats are the slowest
Why are proteases like pepsin secreted as inactive zymogens rather than in their active form?
Click to reveal answer
To prevent self-digestion. Active proteases would digest the cells that produce them. By secreting inactive zymogens (pepsinogen, trypsinogen, etc.), the enzyme only becomes active after it reaches the lumen, where it can safely digest food without harming the secreting tissue.
A patient with autoimmune destruction of parietal cells would develop deficiencies in which two substances?
Click to reveal answer
HCl and intrinsic factor. Loss of parietal cells means no HCl (impaired pepsinogen activation and protein digestion) and no intrinsic factor (impaired B12 absorption in the ileum, leading to pernicious anemia).